MDM
Multi-dimensional microscopy (MDM) is a new technology development project using combined non-linear (i.e., second harmonic generation [SHG], two photon flourescence [TPF], and coherent anti-Stokes Raman scattering [CARS]) and linear (i.e., optical coherence microscopy [OCM]) optical signals. A dual-laser platform will be developed that is capable of operating in multiple simultaneous or sequential scanning modes. MDM will be applied to high-resolution intravital imaging problems, with particular emphasis on the origins of endogenous signals from cells and extracellular matrix. The introductoin of CARS allows for quantitative co-registration of biochemical composition, particularly lipid and water, and the MDM platform will be used to form dynamic images baes on these contrast elements, as well as coherent scattering structures. This core builds on our extensive experience in Optical Coherence Tomography (OCT) and Multi-Photon Microscopy (MPM), and our combined MPM-OCT intravital microscope.
