MPM
Multi-photon microscopy (MPM) uses near infrared (NIR) light to generate two-photon excited fluorescence (TPEF) from UV/VIS absorbing molecules and proteins and/or second harmonic singals (SHG) from structures without a center of symmetry. The reduced scattering at NIR wavelength allows submicron visualization of cells and tissues at depths of several hundred microns with minimal (femtoliter) damage to the living systems. MPM can produce highly detailed images providing information on general tissue morphology, redox state (by means of NAD(P)H, flavins, or degree of indoleamine oxidation) and subresolution determination of collagen fibril orientation and width (through SHG directionality). MPM is a promising clinical diagnostic tool in cancer, wound healing, analysis of cornea and arterial tissue, and in determing protein-protein interactions.
