Laser Microbeam and Medical Program    »     People  |   Education & Dissemination  |   Research & Resources  |   Publications
LAMMP Technology Cores

Multimodality Endoscopic Technologies

Multiphoton microscopy(MPM) is based on multiphoton excited fluorescence and/or harmonic generation and provides molecular contrast and specificity.


(a) OCM and (b) SHG images of a thin slice of fixed rabbit heart stained with hematoxylin and eosin.
(c) OCM and (d) SHG images of a rat tail tendon. Scale bar: 50 µm.

Coherent anti-Stokes Raman scattering(CARS) enables label-free imaging of various important bio-molecules at submicron resolution and at fast image acquisition rates. Our fiber-delivered probe enables patient-friendly CARS examination of superficial tissues. the probe design incorporates separate fibers for excitation light delivery and for signal detection, to suppress the strong non phase-matched four-wave-mixing (FWM).


CARS images of thick tissue samples ex vivo at 2842 cm-1.(a) Small adipocytes of mouse ear skin. (b) Adipocytes of subcutaneous layer of rabbit skin tissue. (c) Meibomian gland in mouse eyelid. Images were acquired in 2s. Scale bar: 50 µm.

LAMMP has played a leading role in the development of fiber-based microendoscopic OCT, MPM, CARS and translated these technologies for in vivo imaging. Although each optical probing technique provides access to relevant diagnostic parameters, integration of several modalities will be necessary to advance the development of a reliable method for diagnosis and management of many complex diseases. An example is cardiovascular diseases such as atherosclerosis. Diagnosis of the latent vulnerability of a plaque lesion relies on tissue structure, chemical compositions, and tissue mechanical properties. Another example is cancer diagnosis. Tissue structure, angiogenesis, and extracellular matrix change are all important parameters for early cancer diagnosis. Although multimodal microscopy systems have been demonstrated by several groups, most reported systems are implemented in a bench top free space microscopy platform, which limits their potential applications for in vivo animal and human patient imaging. LAMMP has pioneered several important multimodal imaging modalities, such as fiber-based combined MPM/OCT system and combined US/OCT endoscopy for intravascular imaging.

Optical Coherence Tomography (OCT) is a non-invasive, non-contact imaging modality based on coherence gating where the short coherence length of a broadband light source is used in an interferometer to obtain micrometer-scale, cross-sectional imaging of biological tissue. The high spatial resolution of the OCT structural image enables noninvasive in vivo "optical biopsy" and provides immediate and localized tissue structure information. Functional OCT (F-OCT) is extension of OCT for functional imaging of tissue physiology to enhance the diagnostic capability of OCT.


(a) One slice and (b) 3-D reconstruction of Human left lower lobe bronchus obtained by OCT.

F-OCT represents the very essence of the NIH technology center program: the technology has been transformed from laboratory into a useful translational clinical research device. Current collaborative projects are driving the need to improve the performance for specific applications in cardiovascular vessel, retina, airway, oral cavity, GI tract, skin, cartilage as well as other tissues.

OCT is a non-invasive, non-contact imaging modality based on coherence gating where the short coherence length of a broadband light source is used in an interferometer to obtain micrometer-scale, cross-sectional imaging of biological tissue. The high spatial resolution of the OCT structural image enables noninvasive in vivo "optical biopsy" and provides immediate and localized tissue structure information. Functional OCT (FOCT) is an extension of OCT for functional imaging of tissue physiology to enhance the diagnostic capability of OCT. All F-OCT has Doppler and Phase resolved Doppler OCT capability. F-OCT fiber-based platforms are available for collaborative projects that image blood vessels, retina, airway, oral cavity, GI tract, skin, cartilage as well as other tissues. There are currently 4 F-OCT instruments available for collaborative studies.

Features commmon to all:
  • Depth resolution: 5-10 µm
  • Lateral resolution: 10 µm
  • Imaging speed: 1- 50 frames/second
  • Sensitivity: 95-108 dB
  • Varying probes for endoscopic clinic imaging: rotational and linear scanning
  • Center wavelength: 1310 nm
  • Functional capability: Structure, blood flow velocity (Doppler) and birefringence (polarization) imaging
Specific features:

TDOCT

  • Rapid scanning optical delay line
  • Cart-based system for transport
  • Generally used for clinical and animal studies (can be shipped offsite)
  • Currently housed in LAMMP

SDOCT

  • Spectrometer system
  • Capable of 3D imaging
  • Imaging at up to 92 frames/second
  • Sensitivity of 105 dB
  • Functional OCT capability
  • Currently housed in LAMMP

SSOCT

  • Swept laser system
  • Imaging at up to 50 frames/second
  • Sensitivity of 106 dB
  • Generally used for clinical and animal studies
  • Currently housed in LAMMP

SSOCT-IVUS

  • Swept laser OCT-IVUS (intravascular ultrasound) multimodality system
  • Imaging at up to 20 frames/second
  • OCT sub-system sensitivity of 108 dB
  • With scanning endoscopic probe OD<0.7 mm, widely used for animal studies
  • Currently housed in LAMMP

FDOCT-MC

  • Swept laser system
  • Imaging at up to 50 frames/second
  • Sensitivity of 106 dB
  • Linear scanning endoscopic probe for use in clinical studies
  • Generally used for clinical studies
  • Currently housed at UCI Medical Center
  • Custom designed endoscopic probes upon request

LROCT

  • Long Range Swept laser system
  • Imaging at up to 50 frames/second
  • Sensitivity of 106 dB, long imaging range with a 6 dB sensitivity roll off at 9.5mm total offset and a 10 dB roll off at 14mm
  • Linear scanning endoscopic probe for use in clinical studies
  • Currently housed in LAMMP

OCE

  • 890nm spectral-domain system
  • Imaging at up to 20 frames/second
  • Enable evaluating the elastic properties of tissue
  • Currently housed in LAMMP

OCM/MPM

  • Multiphoton microscopy (MPM) with Fourier domain optical coherence microscopy (OCM) multimodality system
  • The fiber laser has a central wavelength of 1040nm and bandwidth of 29 nm. Longer excitation wavelength is used to increase penetration depth and increase the excitation efficiency
  • With a handheld probe
  • Currently housed in LAMMP

CARS

  • Pump beam 750 -920 nm Stock beam 1064 nm at 76 MHz, matching the Raman shift of the CH2 stretch in lipids
  • With scanning endoscopic probe, generally used for animal studies
  • Currently housed in LAMMP

Zhongping Chen Ph.D.
Professor
Phone: 949.824.1247
Email: z2chen@uci.edu

Jun Zhang Ph.D.
Assistant Researcher
Phone: 949.824.3390
Email: junzhang@uci.edu

Gangjun Liu Ph.D.
Postdoctoral Researcher
Phone: 949.824.4713
Email: gangjunl@uci.edu

GRADUATE STUDENTS
Joseph Jing
Jiawen Li
Wenjuan Qi

J. Yin, X. Li, J. Jing, J. Li, D. Mukai, S. Mahon, A. Edris, K Hoang, K. K. Shung, M. Brenner, J. Narula, Q. Zhou and Z. Chen
Novel combined miniature optical coherence tomography ultrasound probe for in vivo intravascular imaging.
J. of Biomedical Optics 16, 060505-1-3 (2011). PMCID 3124531

M. S. Mathews, J. Su, E. Heidari, E. I. Levy, M. E. Linskey and Z. Chen
Neuroendovascular optical coherence tomography imaging and histological analysis.
Neurosurgery 69, 430-439 (2011). PMCID 3337214

Y.-C. Ahn, J. Chung, P. Wilder-Smith and Z. Chen
Multimodality approach to optical early detection and mapping of oral neoplasia.
J. Biomedical Optics 16, 076007 (2011). PMCID 3146547

S. Moon, G. Liu and Z. Chen
Mode-filtered large-core fiber for short-pulse delivery with reduced nonlinear effects.
Optics Letters 36, 3362-3364 (2011). PMCID 3368707

C. Robertson, S.-W. Lee, Y.-C. Ahn, S. Mahon, Z. Chen, M. Brenner and S. C. George
Investigating in vivo airway wall mechanics during tidal breathing with optical coherence tomography.
J. of Biomedical Optics 16, 106011 (2011). PMCID 3210193

W. Wei, Z. Li, Q. Zhou, K. K. Shung and Z. Chen
Integrated ultrasound and photoacoustic probe for co-registered intravascular imaging.
J. of Biomedical Optics 16, 106001 (2011). PMCID 3206921

J. Zhang, J. Jing, P. Wang and Z. Chen
Polarization-maintaining buffered Fourier domain mode-locked swept source for optical coherence tomography.
Optics Letters 36, 4788-4790 (2011). PMCID 3337216

G. Liu, W. Jia, V. Sun, B. Choi and Z. Chen
High-resolution imaging of microvasculature in human skin in-vivo with optical coherence tomography.
Optics Express 20, 7694-7705 (2012). PMCID 3368711

J. S. Park, Z. Chen, M. Y. Jeong and C.-S. Kim
Double common-path interferometer for flexible optical probe of optical coherence tomography.
Optics Express 20, 1102-1112 (2012). NIHMS 380643



View all LAMMP Technology Cores

Research

Contact Us

  • Hanna Kim
    Resource Coordinator
    Phone:949.824.2251
    Email: hhkim3@uci.edu

Supported by


P41EB015890