SEMINARS IN BIOMEDICAL ENGINEERING

Clinical applications of multiphoton microscopy

Magnus Lilledahl, PhD

This presentation will be an overview of ongoing projects at the Norwegian University of Science and Technology (NTNU) using multiphoton microscopy (MPM) for characterizing tissue structures. The goal of the presentation is to lay the groundwork for potential collaboration between NTNU and The Beckman laser institute in the field of multi-photon microscopy (MPM).

Our main focus areas can be divided into three parts, (1) Characterization of (mostly) cardiovascular structures using MPM, (2) quantitative analysis of MPM images and (3) relating microstructure to macroscopic mechanical properties. We are using MPM to characterize cardiovascular structures like vulnerable plaque, aneurysms, normal vessel wall and heart valves. For these images to be valuable, it is necessary to have automated analysis methods to extract relevant parameters characterizing the structure of the connective fibers, e.g. direction, thickness, density and type. Mechanical properties are important in many clinical applications and biomechanical models are used to understand the behavior of tissue. Knowing the structure of the connective tissue and how it behaves under applied stress is valuable for improving existing biomechanical models. This is very relevant for characterizing cardiovascular structures and other structures, e.g. cartilage.

[ Back ]

sponsored by

The Laser Microbeam and Medical Program (LAMMP)
a NIH biotechnology resource facility at the Beckman Laser Institute