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Elucidating biological processes using second harmonic generation microscopy

Magnus Lilledhal, PhD

Second harmonic generation microscopy has been around for a while and can relatively easily provide information about collagen structure which is more difficult to access with other methods. Still, the majority of researchers that study tissues and diseases where collagen is a key element has never heard of SHG. It is more challenging to interpret coherent nonlinear signals as compared to conventional confocal microscopy and thus a very close collaboration between physicists and biomedical researchers are needed. In this talk, I will show some examples of applications were SHG provides important biological information to better understand normal and pathological physiology, information which is not readily available with other methods. The main area of applications is cartilage but I will also touch upon applications in cardiovascular disease and cancer

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