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Multi-scale histopathology by label-free optical imaging

Woonggyu Jung, Ph. D

The histological optical imaging is a gold standard method to observe the biological tissues, which follows routine process such as dissection, embedding, sectioning, staining, visualization and interpretation of specimens. This technique has a long history of development, and is used ubiquitously in pathology, despite being highly time and labour-intensive. Advanced optical imaging techniques developed over the last decade have enabled to provide high sensitivity, high resolution and non-invasive biological information. However, acquiring high throughput, large volume tissue anatomy remains a difficult challenge due to the effect of light scattering, which limits the penetration imaging depth and lateral resolution. Recently, various optical imaging methods have been introduced to create volumetric anatomy data of ex vivo tissues using physical tissue sectioning or optical clearing. Even though these new approaches present the distinguished volumetric anatomy in various scales, they are still not suitable for use in statistical studies with multiple tissues and organs.
Here, we introduce novel label-free and multi-scale imaging modality based on serial optical coherence microscopy (OCM). OCM is a potential technique to build volumetric anatomy of mouse tissues or organs due to its simplicity, efficiency, robustness, and high-throughput capabilities. This presentation covers the latest work of large-scale brain and kidney imaging using OCM and its potential in bio-applications. Specifically, the talk will highlight other label-free optical imaging modalities including wide-field quantitative phase microscopy and optical projection tomography toward to multi-scale histopathology.

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